Under isoflurane anesthesia, one hind limb was secured and a transcutaneous needle electrode inserted at the ankle for tibial nerve stimulation. Two recording electrodes were inserted into hind paw interosseous muscles. Stimulus generation and recording of M and H waves from the resulting electromyogram were performed using a Powerlab 4/30 connected to a computer running Scope software (AD Instruments, Colorado Springs, CO). Tibial nerve stimulation used bursts of 5 × 200 μs duration square waves with 40-μs interpulse intervals. Each burst was repeated at 1 Hz stimulation frequency, which, in normal rats, causes an ∼40% decrease in H-wave amplitude between the first and subsequent bursts (15 (link)). Stimulation intensity was increased by 0.125-V increments until the stimulus that produced the maximum H-wave amplitude (Hmax) was found. RDD was calculated as percentage change in H-wave amplitude evoked by the second (H2) compared with the first (H1) stimulation burst. In rats, H2 is representative of all subsequent responses (14 (link)).
Protocol detail
Measuring Rat Tibial Nerve Reflex
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Weill Cornell Medical College in Qatar
Other organizations : Manchester Academic Health Science Centre, Manchester Royal Infirmary, National Health Service, University of California, San Diego, University of Exeter, Queensland University of Technology
Top 5 similar protocols
Variable analysis
independent variables
- Stimulation intensity (increased by 0.125-V increments)
- H-wave amplitude
- RDD (percentage change in H-wave amplitude evoked by the second (H2) compared with the first (H1) stimulation burst)
- Isoflurane anesthesia
- Tibial nerve stimulation (bursts of 5 × 200 μs duration square waves with 40-μs interpulse intervals, repeated at 1 Hz stimulation frequency)
- Placement of transcutaneous needle electrode at the ankle for tibial nerve stimulation
- Placement of two recording electrodes into hind paw interosseous muscles
- Stimulus generation and recording using a Powerlab 4/30 connected to a computer running Scope software
- Normal rats, where 1 Hz stimulation frequency causes an ∼40% decrease in H-wave amplitude between the first and subsequent bursts
- Not mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!