Pure enzyme reaction: A total of 1 mL system containing 12 mM p-nitrobenzaldehyde, 48 mM nitromethane, 0.3 mg/mL pure enzyme and solvent water, was reacted at 30 °C in a metal bath for 10 min. 10 μL of the reaction solution was taken and diluted 50 times with isopropanol and filtered through 0.25 μm organic filter membranes. HPLC was used for analysis, and the yield was calculated using formula:
Enzymatic Synthesis of Nitro-Aromatic Compound
Pure enzyme reaction: A total of 1 mL system containing 12 mM p-nitrobenzaldehyde, 48 mM nitromethane, 0.3 mg/mL pure enzyme and solvent water, was reacted at 30 °C in a metal bath for 10 min. 10 μL of the reaction solution was taken and diluted 50 times with isopropanol and filtered through 0.25 μm organic filter membranes. HPLC was used for analysis, and the yield was calculated using formula:
Corresponding Organization :
Other organizations : Zhejiang University
Variable analysis
- Crude enzyme reaction: Crude enzyme
- Pure enzyme reaction: 0.3 mg/mL pure enzyme
- Yield (%) calculated using the formula: [product]/[substrate]int × 100%
- Crude enzyme reaction: 12 mM p-nitrobenzaldehyde, 48 mM nitromethane, solvent water, 30 °C, 10 min
- Pure enzyme reaction: 12 mM p-nitrobenzaldehyde, 48 mM nitromethane, solvent water, 30 °C, 10 min
- Crude enzyme reaction: No positive or negative controls specified.
- Pure enzyme reaction: No positive or negative controls specified.
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