The overall secondary structures of purified proteins were investigated at 25 °C using a J-810 circular dichroism (CD) spectropolarimeter (Jasco, Hachioji, Tokyo, Japan). CD spectra were collected from 170 to 270 nm at a scanning rate of 200 nm/min with a path length of 0.1 cm (Figueroa et al., 2016 (link); Giudice et al., 2017 (link)).
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