Floxed G9a (G9aflox/flox) mice were generated as described previously10 (link),46 (link) and Alb-Cre mice were purchased from The Jackson Laboratory47 (link). Mice were maintained in a temperature- and light-controlled facility, and permitted ad libitum regular chow diet and autoclaved water. All mice were backcrossed with the C57BL/6 strain and the male progeny were analyzed. All the experiments were performed in accordance with protocols approved by the Animal Ethics Committee of the University of Tokyo.
HCC was induced as previously described23 (link). 15-day-old WT and G9aΔHep mice were injected intraperitoneally (i.p.) with DEN (Sigma, St. Louis, MO, USA) (25 mg/kg) alone or in combination with 22 weekly injections of CCl4 (Wako, Osaka, Japan) (0.5 ml/kg i.p., dissolved in corn oil). To evaluate acute effects of G9a in damaged hepatocytes, 8-week-old WT and G9aΔHep mice were treated with DEN (100 mg/kg i.p.) and sacrificed 48 h after DEN administration. In vivo G9a inhibitor treatment was performed as previously described48 (link). WT mice were injected i.p. with G9a inhibitor UNC0642 (5 mg/kg) for 10 days before and after DEN administration. Randomization or blinding of animal experiments were not possible.
HCC was induced as previously described23 (link). 15-day-old WT and G9aΔHep mice were injected intraperitoneally (i.p.) with DEN (Sigma, St. Louis, MO, USA) (25 mg/kg) alone or in combination with 22 weekly injections of CCl4 (Wako, Osaka, Japan) (0.5 ml/kg i.p., dissolved in corn oil). To evaluate acute effects of G9a in damaged hepatocytes, 8-week-old WT and G9aΔHep mice were treated with DEN (100 mg/kg i.p.) and sacrificed 48 h after DEN administration. In vivo G9a inhibitor treatment was performed as previously described48 (link). WT mice were injected i.p. with G9a inhibitor UNC0642 (5 mg/kg) for 10 days before and after DEN administration. Randomization or blinding of animal experiments were not possible.
Free full text: Click here
