NCI-H660 cells (obtained directly from ATCC, CRL-5813) were maintained in HITES media (ATCC) with 5% fetal bovine serum (Atlanta Biologicals) in humidified Steri-Cult CO2 incubators (Thermo Scientific). LuCaP patient-derived xenograft tumors (established in-house; [12 (link)]) were harvested and dissociated using the human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s protocol.
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