Maintaining NCI-H660 Cells and Dissociating LuCaP Tumors
Corresponding Organization : Fred Hutch Cancer Center
Variable analysis
- Cell line used: NCI-H660 cells (obtained directly from ATCC, CRL-5813)
- Tumor samples used: LuCaP patient-derived xenograft tumors (established in-house; [12 (link)])
- Not explicitly mentioned
- Media used: HITES media (ATCC) with 5% fetal bovine serum (Atlanta Biologicals)
- Incubation conditions: humidified Steri-Cult CO2 incubators (Thermo Scientific)
- Tumor dissociation method: human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer's protocol
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
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