Profiling miRNA Expression in Treatment Outcomes

Blood was collected before treatment in PAXgene and total RNAs, including small RNAs, were extracted as previously described (22 (link)). RNA quality was assessed using the 2100 Bioanalyzer assay (Agilent, Les Ulis, France), and according to the criteria of the Minimum Information for publication of Quantitative real-time PCR Experiments MIQE guidelines, only samples with a RIN>8 were used. Sixteen patients were analyzed with the TaqMan Low-Density Array (TLDA) technology as previously described (22 (link)) and divided in two groups according to treatment response outcome. One group was composed of 8 patients with CR with blood and BM uMRD. The other group was composed of 8 patients with no-CR and detectable MRD. Validation of miRNA expression levels were quantified in a second cohort of 100 treatment-naive patients using multiplex Taqman microRNA assays (Life Technologies, Saint Aubin, France) as previously described (22 (link)).