The enzymatic activity of caspase-3/7 was determined using the Caspase-Glo 3/7 assay kit following the manufacturer’s instructions (Promega, Madison, WI, United States), as previously reported (Konno et al., 2014 (link)). Briefly, 3 × 103 cells were plated in triplicates in 96-well plates and transfected as indicated. An equal amount of Caspase-Glo 3/7 substrate was added to the culture and subsequently incubated for 3 h. Following incubation, caspase-3/7 activities were evaluated as an indicator of cell apoptosis using a GloMax-96 Microplate luminometer (Promega). The results were shown as the fold change relative to the control cells.
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