Cell invasion assay was performed as previously described [22 (link)]. Briefly, the upper chamber of the trans-well membrane was coached with Matrix gel (BD). MCF-7-CT45A1 breast cancer cells were pre-treated with SP1 and SULF2 inhibitors for 72 hours. The cells were counted, and alive cells re-suspend in serum-free DMEM without inhibitors and 2 × 104 cells were seeded into the upper chamber in 200 μL medium. Then 500 μL DMEM (10% FBS) was added to the low chamber and cultured for 24 hours. The cells on bottom of the membrane of the chamber were stained by Wright-Giemsa solution and photographed. Three randomly chosen fields were analyzed for each trans-well.
Protocol detail
Breast Cancer Cell Invasion Assay
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Nantong University, Soochow University
Top 5 similar protocols
Variable analysis
independent variables
- SP1 inhibitor
- SULF2 inhibitor
- Cell invasion
- Serum-free DMEM (without inhibitors)
- DMEM (10% FBS)
- Incubation time (24 hours)
- Cell count (2 × 10^4 cells)
- Positive control: MCF-7-CT45A1 breast cancer cells without any inhibitor treatment
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!