Collection methods
for CD spectra were previously described and adapted here.27 (link) In short, the stock solutions of wild-type TsaM
and TsaM variants were used to make 350 μL samples by diluting
to 10 μM using a buffer containing 5 mM HEPES (pH 8.0) and 20
mM NaCl. The prepared samples were transferred into a 10 mm quartz
cuvette (Hellma), and the CD spectra were measured using a Jasco J-1500
CD spectrometer. Each sample spectrum was an average of three cumulative
spectra.
for CD spectra were previously described and adapted here.27 (link) In short, the stock solutions of wild-type TsaM
and TsaM variants were used to make 350 μL samples by diluting
to 10 μM using a buffer containing 5 mM HEPES (pH 8.0) and 20
mM NaCl. The prepared samples were transferred into a 10 mm quartz
cuvette (Hellma), and the CD spectra were measured using a Jasco J-1500
CD spectrometer. Each sample spectrum was an average of three cumulative
spectra.
