Vesicle preparations were fixed and stained as described (Bouchareychas et al., 2020 (link)) with minor alterations. The uranyl‐oxalate (step 6) was substituted with 2% of uranyl‐acetate, and the ratio of methylcellulose to uranyl‐acetate was changed to 1% each (step 7). Grids were analysed using Tecnai Spirit BioTWIN electron microscope (FEI, Netherlands) at 120 kV. Pictures were taken with an eagle bottom‐mount CCD camera and processed with ImageJ (NIH).
Bittel M., Reichert P., Sarfati I., Dressel A., Leikam S., Uderhardt S., Stolzer I., Phu T.A., Ng M., Vu N.K., Tenzer S., Distler U., Wirtz S., Rothhammer V., Neurath M.F., Raffai R.L., Günther C, & Momma S. (2021). Visualizing transfer of microbial biomolecules by outer membrane vesicles in microbe‐host‐communication in vivo. Journal of Extracellular Vesicles, 10(12), e12159.
Corresponding Organization : Goethe University Frankfurt
Other organizations :
Universitätsklinikum Erlangen, Northern California Institute for Research and Education, University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz
Uranyl-oxalate substituted with 2% of uranyl-acetate
Ratio of methylcellulose to uranyl-acetate changed to 1% each
dependent variables
Vesicle morphology and structure observed under electron microscope
control variables
Vesicle preparations were fixed and stained as described in the referenced publication (Bouchareychas et al., 2020)
Grids were analyzed using Tecnai Spirit BioTWIN electron microscope at 120 kV
Pictures were taken with an eagle bottom-mount CCD camera and processed with ImageJ (NIH)
controls
None explicitly mentioned
Annotations
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