Optical-electrophysiology was performed as outlined previously73 (link) using a voltage sensitive FluoVolt dye (Thermo Fisher Scientific, USA), and the absolute fluorescence signals were obtained with a custom built novel high-throughput imaging platform (OptioQUANT) (Ternion Bioscience, Singapore). All data were analyzed with Igor Pro (WaveMetrics, USA). AP durations of WT and Mfn 1&2 KO mouse cardiomyocytes were measured at 30%, 50%, 70% and 90% decrement of AP amplitude and analyzed. Cells which exhibited irregular events during acquisition were excluded from analyses. All values are given as mean ± SEM.
Kwek X.Y., Hall A.R., Lim W.W., Katwadi K., Soong P.L., Grishina E., Lin K.H., Crespo-Avilan G., Yap E.P., Ismail N.I., Chinda K., Chung Y.Y., Wei H., Shim W., Montaigne D., Tinker A., Ong S.B, & Hausenloy D.J. (2022). Role of cardiac mitofusins in cardiac conduction following simulated ischemia–reperfusion. Scientific Reports, 12, 21049.
Other organizations :
National Heart Centre Singapore, University College London, National University of Singapore, Chinese University of Hong Kong, Naresuan University, KK Women's and Children's Hospital, Singapore Institute of Technology, Institut Pasteur, European Genomic Institute for Diabetes, Institut Pasteur de Lille, Centre Hospitalier Universitaire de Lille, Université de Lille, Inserm, William Harvey Research Institute, Queen Mary University of London
Cells which exhibited irregular events during acquisition were excluded from analyses
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
