Western Blot Analysis of Stem Cell Markers

Western blots were performed as described previously 24 (link) using antibodies specific for Bmi1, SOD2, Slug (Cell Signaling Technology, Beverly, MA, USA), Nanog (Aviva Systems Biology, San Diego, CA, USA), C-myc, ABCG2 (ATP-binding cassette transporters) (Santa Cruz, Dallas, Texas, USA) and GAPDH (Sigma, San Louis, MO, USA). The results obtained from western blot were quantified by Quantity One software (Bio-Rad) and shown in Supplementary Material: Figure S2.

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He Q., Liu Z., Zhao T., Zhao L., Zhou X, & Wang A. (2015). Bmi1 Drives Stem-Like Properties and is Associated with Migration, Invasion, and Poor Prognosis in Tongue Squamous Cell Carcinoma. International Journal of Biological Sciences, 11(1), 1-10.

Publication 2015

C-myc ABCG2 GAPDH Quantity One software

Antibodies Atp binding cassette transporters Bmi1 C myc Gapdh Slug Sod2 Western blot Western blots

Corresponding Organization :

Other organizations : Sun Yat-sen University, University of Illinois Chicago

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Variable analysis

independent variables

Antibodies specific for Bmi1, SOD2, Slug, Nanog, C-myc, ABCG2 (ATP-binding cassette transporters)

dependent variables

Protein expression levels of Bmi1, SOD2, Slug, Nanog, C-myc, ABCG2 (ATP-binding cassette transporters)

control variables

GAPDH

controls

Positive control: Not specified
Negative control: Not specified

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