Total RNA was isolated from primary hepatocytes, pancreatic islets, and hippocampal neurospheres, treated with 0.1% DMSO (control) or FK866 (200 nM for primary hepatocytes, and 10 nM for pancreatic islets and hippocampal neurospheres). Primary hepatocytes and pancreatic islets were isolated from 3 month-old C57BL/6J (B6) male mice (Jackson Laboratories). To determine transcriptional changes induced by FK866 treatment, microarray analyses were conducted using the Illumina Mouse Ref 8 whole genome microarrays (version 2). The background-subtracted raw microarray data were subjected to Z score transformation, and Z ratios were calculated as described previously 5 (link). All data were analyzed by the R statistical software package.
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Transcriptomic Profiling of FK866 Effects
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Other organizations : Washington University in St. Louis, University of Genoa, USA Mitchell Cancer Institute, University of South Alabama
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Variable analysis
independent variables
- Treatment with 0.1% DMSO (control) or FK866 (200 nM for primary hepatocytes, and 10 nM for pancreatic islets and hippocampal neurospheres)
- Transcriptional changes induced by FK866 treatment, as measured by microarray analysis
- Primary hepatocytes and pancreatic islets were isolated from 3 month-old C57BL/6J (B6) male mice (Jackson Laboratories)
- Positive control: Untreated primary hepatocytes, pancreatic islets, and hippocampal neurospheres
- Negative control: Primary hepatocytes, pancreatic islets, and hippocampal neurospheres treated with 0.1% DMSO
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