Venous Remodeling Histomorphometric Analysis

We analyzed 3–5 outflow veins per animal per group for histomorphometric analysis. Sections were stained for Hematoxylin and Eosin (H and E) to assess venous remodeling. Images were digitized to capture a minimum of 1936 × 1460 pixels covering one entire cross-section utilizing an M2 Microscope (Carl Zeiss) with an Axiocam 503 color camera (Carl Zeiss). These slides were analyzed using ZEN 2 blue edition version 2.0 (Carl Zeiss) as described elsewhere^{4 (link),11 (link),16 (link),30 (link)}. We measured the lumen vessel area, neointima, media, and adventitia areas, along with cell density in each of the layers.

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Cai C., Kilari S., Zhao C., Singh A.K., Simeon M.L., Misra A., Li Y., Takahashi E., Kumar R, & Misra S. (2021). Adventitial delivery of nanoparticles encapsulated with 1α, 25-dihydroxyvitamin D3 attenuates restenosis in a murine angioplasty model. Scientific Reports, 11, 4772.

Publication 2021

M2 Microscope Axiocam 503 color ZEN 2 blue edition version 2.0

Adventitia Animal Eosin Microscope Neointima Venous Vessel

Corresponding Organization :

Other organizations : Union Hospital, Huazhong University of Science and Technology, Mayo Clinic, Mayo Clinic in Arizona

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Lumen vessel area
Neointima area
Media area
Adventitia area
Cell density in each of the layers

control variables

Not explicitly mentioned

controls

Positive controls: Not mentioned
Negative controls: Not mentioned

Annotations

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