The HB0801 strain was isolated from the lesioned lung of an infected beef cattle from Yingcheng city in Hubei province, China by this laboratory [4] and stored at the China Center for Type Culture Collection (CCTCC # M2010040) at Wuhan University, Wuhan, China. HB1007 was isolated from milk of a dairy cow with mastitis in Hubei in 2010.
The strain was cultured on a pleuropneumonia-like organisms (PPLO) agar plate at 37°C, in a 5% CO2 atmosphere for 3 days or in PPLO broth (2.5 g glucose, 10.5 g PPLO, 2.5 g yeast, 50 mL donor equine serum (Thermo Fisher Scientific, Waltham, MA, USA), 5 mL10% arginine, 5 mL 10×MEM, 5 mL of 80,000 IU/mL penicillin-G, and 500 µL 1% phenol red) at 37°C for 3 days on an orbital shaker.
The strain was cultured on a pleuropneumonia-like organisms (PPLO) agar plate at 37°C, in a 5% CO2 atmosphere for 3 days or in PPLO broth (2.5 g glucose, 10.5 g PPLO, 2.5 g yeast, 50 mL donor equine serum (Thermo Fisher Scientific, Waltham, MA, USA), 5 mL10% arginine, 5 mL 10×MEM, 5 mL of 80,000 IU/mL penicillin-G, and 500 µL 1% phenol red) at 37°C for 3 days on an orbital shaker.
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