Knockdown of Mdka in Zebrafish Embryos

Morpholino oligonucleotides (MOs; Gene Tools, LLC, Cowallis, OR, USA) were used to knockdown Mdka in embryos. Previously described and validated protocols for the ATG-targeted mdka MOs were used (see [44 (link)]). Embryos were injected at the 1-cell stage with 3ng of Mdka MO (5’-CCGCATTTTGTTTTCTGTGTCGAAA-3’) or mismatch control (Mdka MM, 5’-CCGgATTTTcTTTTCTcTcTgGAAA-3’) diluted in 1x Danieau buffer [45 (link)], and analyzed at 48 hours post fertilization (hpf).

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Gramage E., D’Cruz T., Taylor S., Thummel R, & Hitchcock P.F. (2015). Midkine-a Protein Localization in the Developing and Adult Retina of the Zebrafish and Its Function During Photoreceptor Regeneration. PLoS ONE, 10(3), e0121789.

Publication 2015

Morpholino oligonucleotides (MOs;

Buffer Cell Embryos Fertilization Gene Morpholino oligonucleotides

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor, Wayne State University, Michigan United

Top 5 similar protocols

Variable analysis

independent variables

Mdka MO (5'-CCGCATTTTGTTTTCTGTGTCGAAA-3')
Mdka MM (5'-CCGgATTTTcTTTTCTcTcTgGAAA-3')

dependent variables

Phenotypic changes at 48 hours post fertilization (hpf)

control variables

Embryo developmental stage (1-cell stage injection)
Injection volume (3ng diluted in 1x Danieau buffer)
Developmental time point (48 hpf analysis)

positive control

Not specified

negative control

Mdka MM (5'-CCGgATTTTcTTTTCTcTcTgGAAA-3')

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