Assessing Fungal Morphological Characteristics

Crops were submerged in a three-level orbital agitation shaker (HD-4000, Actum, Medellín, Colombia), with fluorescent tube lamps (15W-T8, 26 mm, 6500 K). Cultures in solid media were grown in a conventional natural convection incubator (WTB Binder, Tuttlingen, Germany).
The length of germ tubes, diameters, and pellet filamentation (Figure 10) were measured via digital image analysis with ImageJ software (National Institutes of Health, Bethesda, Maryland. USA). The micrographs were obtained with a digital sensor (16 MP, f/1.8, 28 mm. Lg Electronics, Yeonji-dong, Busan, South Korea), coupled to a conventional optical microscope (Alphaphot-2 YS2, Nikon, Tokio, Japan) [33 (link),34 (link)].
The data obtained were stored in the Microsoft Excel 2016 office suite (Microsoft, Redmond, Washington, US) and processed through Statgraphics Centurion XVI Version 16.2.04 (Statgraphics Technologies Inc. The Plains, Virginia, US) and SPSS Statistics V23.0 (IBM, Armonk, Nueva York, US).