Fungal Protein Extraction and Analysis

Fungal mycelia were collected by filtration and then were frozen in liquid nitrogen and ground into a fine powder with a mortar and pestle [18 (link)]. Protein was extracted with the lysis buffer described previously [19 (link)]. Protein concentration was determined by the Bradford assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA), and western blot analysis was performed using standard procedures. Antibodies used in this study include anti-H3K14ac (ab52946) and anti-H3 antibodies (ab1791) (Abcam, Cambridge, MA, USA).

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Tang P., Han J.J., Zhang C.C., Tang P.P., Qi F.N., Zhang K.Q, & Liang L.M. (2023). The Growth and Conidiation of Purpureocillium lavendulum Are Co-Regulated by Nitrogen Sources and Histone H3K14 Acetylation. Journal of Fungi, 9(3), 325.

Publication 2023

Bradford assay ab52946 ab1791

Anti antibodies Antibodies Assay Buffer Filtration Frozen Fungal mycelia Nitrogen Powder Protein Western blot analysis

Corresponding Organization : Yunnan University

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Variable analysis

independent variables

Extraction method: Protein was extracted with the lysis buffer described previously [19 (link)].

dependent variables

Protein concentration: Protein concentration was determined by the Bradford assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
Protein expression: Western blot analysis was performed using standard procedures.

control variables

Fungal mycelia collection: Fungal mycelia were collected by filtration and then were frozen in liquid nitrogen and ground into a fine powder with a mortar and pestle [18 (link)].

positive controls

Anti-H3 antibodies (ab1791) (Abcam, Cambridge, MA, USA)

negative controls

Not specified

Annotations

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