Isolation of DON-Biotransforming Microbes

DON-biotransforming microorganisms were isolated from soil samples collected from a wheat field at Huazhong Agricultural University in Wuhan, China, where there are frequent FHB epidemics. DON was purified from F. graminearum 52701^{2 (link)} as previously described^{9 (link)}. Standard chemicals such as DON, 3A-DON, NIV, ZEN, α-ZOL, β-ZOL, GO and MG were purchased from SIGMA (St Louis, MO, USA). MM supplemented with DON as a carbon source was used to screen microbial cultures for DON biotransformation capability^{15 (link)}. Nutrient agar (NA) and 10-fold-diluted NA plates were used to isolate single colonies. Wheat (Triticum aestivum) cv. Sumai 3 was used for seedling inoculation.