Comprehensive Western Blotting Methodology

Western blotting was performed following procedures described previously [25 (link)]. The following primary antibodies were used: anti-WNT5A (1:1000, bs-1948R, BIOSS), anti-FLAG (1:1000, 66008-3-Ig, Proteintech), anti-HDAC7 (1:1000, #33418, CST), anti-SNAIL (1:1000, 13099-1-AP, Proteintech), anti-E-cadherin (1:1000, #14472, CST), anti-SLUG (1:1000, #9585, CST), anti-vimentin (1:1000, 10366-1-AP, Proteintech), anti-TWIST1 (1:1000, 25465-1-AP, Proteintech), anti-HDAC1 (1:1000, #5356, CST), anti-HDAC2 (1:1000, #5113, CST), anti-HDAC3 (1:1000, #3949, CST), anti-HDAC4 (1:1000, 17449-1-AP, Proteintech), anti-HDAC5 (1:1000, 16166-1-AP, Proteintech), anti-HDAC6 (1:1000, 12834-1-AP, Proteintech), anti-HDAC8 (1:1000, E-AB-14127, Elabscience), anti-HDAC9 (1:1000, 67364-1-Ig, Proteintech), anti-HDAC10 (1:1000, 24913-1-AP, Proteintech), anti-HDAC11 (0.3 µg/mL, PA5-79351, Thermo), and anti-β-actin (1:1000, #3700, CST). The secondary antibodies used were HRP-linked anti-IgG antibodies (1:5000, Zhongshan Company, Beijing, China).