Chromatin Immunoprecipitation (ChIP) Protocol
Corresponding Organization : University of California, Davis
Variable analysis
- 37% w/v formaldehyde crosslinking
- 2.5M glycine quenching
- Sonication in Bioruptor
- Primary antibodies: HDAC1, HDAC2, pan-H4ac, H3K9Ac, H3K9me3, SIN3A
- Enrichment of HDAC1, HDAC2, pan-H4ac, H3K9Ac, H3K9me3, SIN3A at target genomic regions
- Number of cells used (2.5 million)
- Washing with ice cold PBS
- Positive controls: None specified
- Negative controls: IgG Rabbit (Cell Signaling Technologies #2729S, 3 or 5μg), IgG Rabbit (Santa Cruz #2027, 3 or 5μg), IgG Mouse (Santa Cruz #2025, 3 or 5μg)
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