Enzyme Activity Assays in Bacillus odoriphaga

100mg B. odoriphaga collected at three developmental stages, respectively, was used to assay the activity of enzymes. There biological replicates were designed for this study. According to the NR annotation of 45 genes selected randomly in the common differential expression of genes among three comparisons, ten DEGs that could be expressed as eight functional enzymes were selected to validate the gene expression profiles. Pectate lyase (PEL) activity and glucose dehydrogenase (GLD) activity were assayed as described by Kovtunovych et al. [28 ] and Fender et al. [29 (link)], respectively. The activity of glutathione s-transferase (GST) was detected according to Glutathione S-Transferase Fluorescent Activity Kit (Enzo life science, USA). The assay of Carboxylesterase (CarE) activity referenced the methods of Zou et al. [30 (link)]. Alpha-Amylase Assay Kit (Abnova, USA), Lipase Activity Assay Kit III (Sigma, USA), Nitric Oxide Synthase Assay Kit (Antibodies-online, Germany), Fatty Acid Synthase assay Kit (Solarbio, China) were used to assayed the activity of alpha-amylase (AA), lipase (Lip), nitric oxide synthase (NOS) and fatty acid synthase (FAS).