Immunophenotyping of Progenitor Cells

For immunophenotyping of isolated progenitor cells and to monitor evolution upon amplification, flow cytometry was performed with a FACS Calibur^TM (Beckton Dickinson, 11, rue Aristide Bergès, ZI des Iles, BP4, 38801 Le Pont de Claix Cedex, France) cytometer. The different phases of the cell cycle were observed after propidium iodide labeling as described [40 (link)], and the flow cytometry histograms were analyzed with the Modfit LT v3.2 software (Verity Software House, Topsham, ME, USA). Cells were labelled with VioBlue-conjugated anti-CD34 antibody, phycoerythrin (PE)-conjugated anti-CD38 antibody, and a lineage mix of fluorescein isothiocyanate (FITC)-conjugated anti-CD3, CD19, CD56, CD13, CD14, and CD11a antibodies (Miltenyi Biotech). For differentiation assay, a complementary labelling was carried out with Brilliant Violet-conjugated anti-CD34 (Beckton Dickinson Pharmingen), FITC-conjugated anti-CD36 (Beckman Coulter, Villepinte, France), PE-conjugated anti-CD235a (GPA) (Beckman Coulter), and allophycocyanin (APC)-conjugated anti-CD71 (Beckman Coulter) antibodies.

Free full text: Click here

Pourcelot E., El Samra G., Mossuz P, & Moulis J.M. (2023). Molecular Insight into Iron Homeostasis of Acute Myeloid Leukemia Blasts. International Journal of Molecular Sciences, 24(18), 14307.

Publication 2023

FACS CaliburTM Modfit LT v3.2 software VioBlue-conjugated anti-CD34 antibody

Allophycocyanin Anti antibody Anti cd3 Antibodies Assay Cd235a Cd71 Cedex Cells Evolution Flow cytometry Fluorescein Iles Isothiocyanate Phycoerythrin Progenitor cells Propidium iodide Violet

Corresponding Organization : Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Other organizations : Centre Hospitalier Universitaire de Grenoble, Centre National de la Recherche Scientifique

Top 5 similar protocols

Variable analysis

independent variables

Different phases of the cell cycle were observed after propidium iodide labeling.

dependent variables

Evolution of progenitor cells upon amplification, as monitored by flow cytometry.
Percentage of cells in different phases of the cell cycle, as determined by the Modfit LT v3.2 software.

control variables

The flow cytometry was performed using a FACS Calibur (Beckton Dickinson, France) cytometer.
Cells were labeled with specific antibodies: VioBlue-conjugated anti-CD34, phycoerythrin (PE)-conjugated anti-CD38, and a lineage mix of FITC-conjugated anti-CD3, CD19, CD56, CD13, CD14, and CD11a.
For the differentiation assay, cells were labeled with Brilliant Violet-conjugated anti-CD34, FITC-conjugated anti-CD36, PE-conjugated anti-CD235a (GPA), and allophycocyanin (APC)-conjugated anti-CD71 antibodies.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!