Egg PC liposomes (extruded to 100 nm, 1 mM), STY-BODIPY (1 μM), and cholesterol (40 μM), desmosterol (40 μM), 7-DHC (40 μM), or vehicle (MβCD) were vortexed in PBS (10 mM, 150 mM NaCl, pH7.4), then 200 μl aliquots were incubated in a black 96-well plates (polypropylene, Nunc) for 20 min at 37 °C. DTUN (200 mM in EtOH) was added to the aliquots. The plate was mixed for 5 min and kinetic data of STY-BODIPYOX was acquired at 488 nm excitation and 518 nm emission by Mithras LB940 microplate reader (Berthold Technologies)29 (link).
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