Benchmarking quanTIseq immune cell profiler
Corresponding Organization : Austrian Drug Screening Institute (Austria)
Other organizations : Universität Innsbruck, Innsbruck Medical University, Leiden University, Vanderbilt University, Vanderbilt University Medical Center, National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg University
Protocol cited in 16 other protocols
Variable analysis
- Normalized microarray data
- Immune cell fractions estimated with flow cytometry, Coulter Counter, or from images of stained tissue slides
- Probe selection (probe with the highest average expression across all samples)
- Mapping of probes to gene symbols
- Immune cell fractions estimated with flow cytometry, Coulter Counter, or from images of stained tissue slides used as ground truth to validate quanTIseq
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