Osteogenic Differentiation of Human BMSCs

Human BMSCs were inoculated at 2.5–4 × 10⁴/cm² in 24-well plates. When the culture reached 100% confluence, the medium was changed to the Human Mesenchymal Stem Cell Osteogenic Differentiation Basal Medium complemented with 10% FBS, 1% Penicillin-streptomycin, 1% Glutamine, 0.2 mM ascorbate, 10 mM β-glycerophosphate, and 0.1 μM dexamethasone (all from Cyagen Biosciences Inc.). Media were changed every two days and the cells were cultured for 14–21 days. The calcium accumulation was assessed using alizarin red sulfate (AR-S; Cyagen Biosciences Inc.) staining and quantified as described previously [12 (link)].

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Wen Q., Zhang S., Du X., Wang R., Li Y., Liu H., Hu S., Zhou C., Zhou X, & Ma L. (2018). The Multiplicity of Infection-Dependent Effects of Recombinant Adenovirus Carrying HGF Gene on the Proliferation and Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells. International Journal of Molecular Sciences, 19(3), 734.

Publication 2018

Human Mesenchymal Stem Cell Osteogenic Differentiation Basal Medium Penicillin-streptomycin Glutamine dexamethasone

Calcium Cells Dexamethasone Glutamine Human Mesenchymal stem cell Osteogenic Penicillin Streptomycin Sulfate β glycerophosphate

Corresponding Organization : Southern Medical University

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Variable analysis

independent variables

Human BMSCs inoculation density (2.5–4 × 10^4/cm^2)

dependent variables

Calcium accumulation (assessed using alizarin red sulfate (AR-S) staining)

control variables

Culture duration (14–21 days)
Culture medium (Human Mesenchymal Stem Cell Osteogenic Differentiation Basal Medium complemented with 10% FBS, 1% Penicillin-streptomycin, 1% Glutamine, 0.2 mM ascorbate, 10 mM β-glycerophosphate, and 0.1 μM dexamethasone)
Media change frequency (every two days)

controls

Not explicitly mentioned
Not explicitly mentioned

Annotations

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