2.5 × 105 flow cytometry-sorted Treg cells were seeded on a 96-well Seahorse cell culture plate and analyzed on a Seahorse XF24 Analyzer (10 (link)). The following drugs and corresponding doses were loaded onto ports A, B, C, and D in the same order: Oligomycin (2.5 uM, Sigma-Aldrich cat no. 75351), CCCP (10 uM, Sigma-Aldrich cat no. C2759), Antimycin A/Piercidin (2 uM each, Sigma-Aldrich cat no. A8674 and 15379, respectively), and 2-deoxyglucose (25 mM, Sigma-Aldrich cat no. D8375).
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Treg Cell Metabolic Profile Analysis
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Corresponding Organization : Lung Institute
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Variable analysis
independent variables
- Oligomycin (2.5 uM)
- CCCP (10 uM)
- Antimycin A/Piercidin (2 uM each)
- 2-deoxyglucose (25 mM)
- Metabolic activity of 2.5 × 10^5 flow cytometry-sorted Treg cells
- 2.5 × 10^5 flow cytometry-sorted Treg cells seeded on a 96-well Seahorse cell culture plate
- Seahorse XF24 Analyzer used for analysis
- No positive or negative controls were explicitly mentioned in the provided information.
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