Mouse Housing and Breeding Protocol

All mice used for breeding and experiments in this study were housed in a light-dark (12 h on/off; lights on at 7:00 AM) and temperature-controlled environment with food and water available ad libitum in the KAIST facilities. Npy-hrGFP mice were obtained from the Jackson laboratory (#006417). For some patch clamp experiments GIRK2 KO mice [41 (link)], used with the permission from Dr. Markus Stoffel (ETH Zurich), were crossed with Npy-hrGFP mice. Agrp-ires-Cre mice (Jackson laboratory, #012899) were crossed with tdTomato reporter mice (Jackson laboratory, #007914), GIRK1^flox/flox mice [22 (link)] or GIRK2^flox/flox mice [23 (link)] for FISH, ISH, IHC, and in vivo metabolic experiments. Mice were fed standard NCD (Teklad global 18% protein 2018S, ENVIGO).