Colorectal Tissue Profiling with IHC

Colorectal tissues (45) and normal colon tissues (N = 46) in a tissue microarray (CO1002b) were provided by US Biomax Inc. (Rockville, MD, USA) through the agency, the Alenabio Inc. (Xi’an, Shaanxi). Hematoxylin-eosin (H&E) and immunocytochemistry (IHC) were performed as previously described [41 (link)]. Antibodies used were the following: DOT1L (ab64077, Abcam), c-Myc (ab32072, Abcam), Ki67 (ab15580), H3K79me1 (ab2886, Abcam), H3K79me2 (ab3594, Abcam), and H3K79me3 (ab2621, Abcam). Signal-positive rate was calculated by using the IHC profiler in the Image J ver.1.46 software.

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Yang L., Lei Q., Li L., Yang J., Dong Z, & Cui H. (2019). Silencing or inhibition of H3K79 methyltransferase DOT1L induces cell cycle arrest by epigenetically modulating c-Myc expression in colorectal cancer. Clinical Epigenetics, 11, 199.

Publication 2019

ab64077 ab32072 ab15580 ab2886 ab3594 ab2621

Antibodies C myc Colon Dot1l Eosin Hematoxylin Immunocytochemistry Microarray Tissue

Corresponding Organization : Southwest University

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Variable analysis

independent variables

Tissue type (colorectal tissues vs. normal colon tissues)

dependent variables

Signal-positive rate for DOT1L, c-Myc, Ki67, H3K79me1, H3K79me2, and H3K79me3

control variables

Tissue microarray (CO1002b) provided by US Biomax Inc. (Rockville, MD, USA) through the agency, the Alenabio Inc. (Xi'an, Shaanxi)
Hematoxylin-eosin (H&E) and immunocytochemistry (IHC) performed as previously described [41 (link)]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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