Enrichment and Transfection of Breast Cancer Cells

BC MCF7 cells were fostered in high-glucose DMEM (Abcam, Shanghai, China) added with 100 U/mL penicillin, 100 mg/L streptomycin, and 10% FBS (Abcam, Shanghai, China) under 5% CO₂, floating in serum-free DMEM/F12 culture medium (Abcam) with 20 ng/mL EGF, 20 ng/mL b-FGF, and 2% B27 (Abcam). Then, we spent 10 days in culturing them within ultra-low attachment plates. miR-30d-5p mimics were synthesized within the GenePharma company (Shanghai, China). Additionally, they were transfected into cells using Lipofectamine 2000 reagent in Invitrogen (USA) in light of the methods of Liu, et al. [16 (link)].

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Zhang L., Ren S., Sang Y., Hu Y., Wang C., Wang X, & Li Y. (2024). miR-30d-5p inhibits proliferation, invasion and migration of breast cancer cells by targeting SERPINE1 and promoting fatty acid β-oxidation. Aging (Albany NY), 16(7), 5856-5865.

Publication 2024

penicillin streptomycin FBS EGF b-FGF Lipofectamine 2000 reagent

Corresponding Organization :

Other organizations : Hebei Medical University

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Variable analysis

independent variables

MiR-30d-5p mimics

dependent variables

Not explicitly mentioned

control variables

High-glucose DMEM (Abcam, Shanghai, China) with 100 U/mL penicillin, 100 mg/L streptomycin, and 10% FBS (Abcam, Shanghai, China) under 5% CO2
Serum-free DMEM/F12 culture medium (Abcam) with 20 ng/mL EGF, 20 ng/mL b-FGF, and 2% B27 (Abcam)
Ultra-low attachment plates

positive controls

Not mentioned

negative controls

Not mentioned

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