Plasma triglycerides (Roche Diagnostics), total cholesterol, free cholesterol, nonesterified FAs (all DiaSys Diagnostic Systems) were quantified using commercially available reagents.
Plasma lipoproteins were separated by fast protein liquid chromatography using a system containing a PU-4180 pump with a linear degasser and UV-4075 UV/VIS detectors (Jasco, Tokyo, Japan), as described (12 (link)). Plasma plant sterols were determined by GC as described (26 (link)). Circulating levels of aspartate aminotransferase, alanine aminotransferase, and albumin were determined using a routine clinical chemistry analyzer (Cobas 6000; Roche Diagnostics) with standard reagents (Roche Diagnostics).
Plasma lipoproteins were separated by fast protein liquid chromatography using a system containing a PU-4180 pump with a linear degasser and UV-4075 UV/VIS detectors (Jasco, Tokyo, Japan), as described (12 (link)). Plasma plant sterols were determined by GC as described (26 (link)). Circulating levels of aspartate aminotransferase, alanine aminotransferase, and albumin were determined using a routine clinical chemistry analyzer (Cobas 6000; Roche Diagnostics) with standard reagents (Roche Diagnostics).
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