ZIKV prME protein-pseudotyped MLV expressing nanoluciferase was generated as previously described [35 (link),38 (link)]. Briefly, HEK293T cells were transfected with pUMVC, pBABE-puro-NanoLuc, and a mammalian vector expressing the ZIKV CaprME. The culture supernatant harvested 2 days after transfection was centrifuged at 1500 × g for 10 min and passed through 0.45 μm filter to remove cell debris. Pseudotyped MLV titre was quantified using qRT-PCR. Packaged viral RNA was extracted using TRIzol LS reagent (Invitrogen) according to the manufacturer’s instructions and subjected to reverse transcription and qRT-PCR using primers targeting the 5′ LTR region of MLV RNA (forward primer 5′-ATTGACTGAGTCGCCCGG-3′ and reverse primer 5′-AGCGAGACCACAAGTCGGAT-3′). To transduce HEK293T cells with the pseudotyped virus, target cells were seeded in a 12-well cell culture plate and inoculated with 0.5 ml media containing pseudotyped virus for 9 h. Cells were then washed and further cultivated in fresh complete media for 48 h prior to measuring reporter activity using a Nano-Glo Luciferase assay kit (Promega) and the GloMax-Multi Detection System (Promega).