Comprehensive Analysis of JUN Expression across Human Cancers

We used the Gene Expression Profiling Interactive Analysis (GEPIA2) [76 (link)] to determine JUN expression in various human cancers, including in brain tumors. Transcripts per million (TPM) values were extracted from different TCGA and Genotype-Tissue Expression (GTEx) datasets, and median gene expression in each cancer and paired normal tissues was calculated and used as an input to R. The following cancers and corresponding healthy tissues were examined: Adrenocortical carcinoma (ACC) and Adrenal Gland; Bladder Urothelial Carcinoma (BCLA) and bladder; Breast invasive carcinoma (BRCA) and breast; Cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) and cervix uteri; Colon adenocarcinoma (COAD) and colon; Lymphoid Neoplasm Diffuse Large B-cell Lymphoma (DLBC) and blood, Esophageal carcinoma (ESCA) and esophagus; Glioblastoma multiforme (GBM) and brain; Kidney Chromophobe (KICH) and kidney; Kidney renal clear cell carcinoma (KIRC) and kidney; Kidney renal papillary cell carcinoma (KIRP) and kidney; Acute Myeloid Leukemia (LAML) and bone marrow; Brain Lower Grade Glioma (LGG) and brain; Liver hepatocellular carcinoma (LIHC) and liver; Lung adenocarcinoma (LUAD) and lung; Lung squamous cell carcinoma (LUSC) and lung; Ovarian serous cystadenocarcinoma (OV) and ovary; Pancreatic adenocarcinoma (PAAD) and pancreas; Prostate adenocarcinoma (PRAD) Prostate, Rectum adenocarcinoma (READ) and colon; Skin Cutaneous Melanoma (SKCM) and skin; Stomach adenocarcinoma (STAD) and stomach; Testicular Germ Cell Tumors (TGCT) and testis; Thyroid carcinoma (THCA) and thyroid; Thymoma (THYM) and blood; Uterine Corpus Endometrial Carcinoma (UCEC) and uterus; Uterine Carcinosarcoma (UCS) and uterus. The JUN mRNA expression profile was compared between tumor samples (TCGA) and paired normal tissues (TCGA normal + GTEx normal), and statistical significance was determined using one-way ANOVA and disease state (Tumor versus healthy tissue of tumor origin).

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Roura A.J., Szadkowska P., Poleszak K., Dabrowski M.J., Ellert-Miklaszewska A., Wojnicki K., Ciechomska I.A., Stepniak K., Kaminska B, & Wojtas B. (2023). Regulatory networks driving expression of genes critical for glioblastoma are controlled by the transcription factor c-Jun and the pre-existing epigenetic modifications. Clinical Epigenetics, 15, 29.

Publication 2023

Acute myeloid leukemia Adenocarcinoma Adrenal gland Adrenocortical carcinoma Anova Bladder Blood Bone marrow Brain Brain tumors Breast Breast carcinoma Cancer gene Cancers Carcinosarcoma Cell Cervical Cervix uteri Chromophobe Colon Colon adenocarcinoma Diffuse large b cell lymphoma Endocervical Esophageal carcinoma Esophagus Gene expression analysis Genotype Glioblastoma multiforme Glioma Human Kidney Kidney carcinoma Kidney clear cell carcinoma Liver Liver carcinoma Lung Lung adenocarcinoma Lymphoid Mrna Ovary Pancreas Prostate Rectum Serous cystadenocarcinoma Skin Skin melanoma Squamous cell carcinoma Stomach Testis Tgct Thymoma Thyroid Thyroid carcinoma Tissue Tumor Tumor origin Urothelial carcinoma Uterine endometrial carcinoma Uterus

Corresponding Organization : Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

Other organizations : Polish Academy of Sciences, Institute of Computer Science

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Variable analysis

independent variables

Cancer type
Tissue type (tumor vs. normal)

dependent variables

JUN mRNA expression (TPM values)

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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