RNA pull-down assay was done as previously described [25 (link)]. Biotin-labeled HCG11 probes and control probes were synthesized by RiboBio. Then, the probes were cultured with cell lysates for RNA enrichment. 30 μL of streptavidin magnetic beads (88816) was added into the samples for precipitation. At last, RNAs bound in the precipitated mixture were eluted and analyzed by qRT-PCR.
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