Endothelial-specific Trpm7 Knockout Mice

Trpm7^fl/fl mice were purchased from the Jackson Laboratory. To generate endothelium-selective deficiency of Trpm7 (Trpm7^ECKO), Trpm7^fl/fl mice were crossed with Tie2-Cre mice. Cre-positive male mice were used for intercrossing to prevent recombination in the female germline. Animal experiments were performed in accordance with guidelines and protocols approved by the Ethics Committee of Xiangya Hospital (No. 2022020390).

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Wu W., Wang X., Liao L., Chen J., Wang Y., Yao M., Zhu L., Li J., Wang X., Chen A.F., Zhang G., Zhang Z, & Bai Y. (2023). The TRPM7 channel reprograms cellular glycolysis to drive tumorigenesis and angiogenesis. Cell Death & Disease, 14(3), 183.

Publication 2023

Endothelium Female Germline Hospital ethics committee Male Mice Recombination Trpm7

Corresponding Organization :

Other organizations : Third Xiangya Hospital, Central South University, Xiangya Hospital Central South University, Hunan University of Traditional Chinese Medicine, XinHua Hospital

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Variable analysis

independent variables

Genotype (Trpm7^fl/fl vs. Trpm7^ECKO)

dependent variables

Not explicitly mentioned

control variables

Sex (Cre-positive male mice used for intercrossing to prevent recombination in the female germline)
Institutional approval (Animal experiments performed in accordance with guidelines and protocols approved by the Ethics Committee of Xiangya Hospital (No. 2022020390))

controls

Positive control: Trpm7^fl/fl mice
Negative control: Not explicitly mentioned

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