Ecell = 4F(ΔZ)(1 − ηcell2)/3(ΔZ1.5)tan θ, where Ecell: Young’s modulus; F: loading force; ηcell: Poisson ratio; ΔZ: indentation; θ: tip half cone opening angle.
Measuring Cellular Biomechanics with AFM
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Corresponding Organization :
Other organizations : Utah State University, University of Kansas, The University of Kansas Cancer Center, University of Kansas Medical Center, Kansas City Kansas Community College
Variable analysis
- Scanning Probe Image Processor (SPIP) software (Image Metrology)
- Young's modulus
- Adhesion force
- Contact mode PicoPlus AFM controlled by Picoview software (Agilent Technologies)
- Medium at room temperature
- Radius of silicon nitride tips: 20 nm
- Spring constant: 0.10-0.11 N m^-1
- Deflection sensitivities: 45-50 nm V^-1
- Half cone-opening angle of tip: 36°
- Cellular Poisson's ratio: 0.5
- Detection time: within 2 hours
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