Data was processed according to the guideline provided by the Class Prediction with Agilent Mass Profiler Professional (Manual part number: 5991-1911EN). The steps included MassHunter Qualitative Analysis and Mass Hunter Profinder to identify untargeted and targeted (recursive) features in the sample data files. This analysis included extraction of molecular features, subtraction of background noise, filtering of data, statistical analysis, database search, and alignment. A compound exchange format file (.CEF) was created for each sample, and downstream analysis was performed using Mass Profiler Professional (MPP) (Agilent Technologies, Santa Clara, CA USA). METLIN Personal Metabolitedatabase28 (link) was then used to identify endogenous and exogenous metabolites.
Protocol detail
Untargeted and Targeted Metabolite Profiling
Data was processed according to the guideline provided by the Class Prediction with Agilent Mass Profiler Professional (Manual part number: 5991-1911EN). The steps included MassHunter Qualitative Analysis and Mass Hunter Profinder to identify untargeted and targeted (recursive) features in the sample data files. This analysis included extraction of molecular features, subtraction of background noise, filtering of data, statistical analysis, database search, and alignment. A compound exchange format file (.CEF) was created for each sample, and downstream analysis was performed using Mass Profiler Professional (MPP) (Agilent Technologies, Santa Clara, CA USA). METLIN Personal Metabolitedatabase28 (link) was then used to identify endogenous and exogenous metabolites.
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Corresponding Organization : Hospital Sungai Buloh
Other organizations : University Kebangsaan Malaysia Medical Centre, National University of Malaysia
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Variable analysis
independent variables
- None explicitly mentioned
- Untargeted and targeted (recursive) features in the sample data files
- Endogenous and exogenous metabolites
- None explicitly mentioned
- Positive controls: Not specified
- Negative controls: Not specified
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