DH5a cells were transformed with PET30a plasmids, and protein was induced with 0.4 mM isopropyl-β-d-thiogalactopyranoside (IPTG) at 16°C for 12 to 16 h. nsp14 was purified with nickel-nitrilotriacetic acid (Ni-NTA) resin (GenScript) as described previously (19 (link), 24 (link)).
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