Himedia Private Limited provided the following chemicals: sodium carbonate, gallic acid, sodium sulphate, copper sulphate, sodium hydroxide, sodium bicarbonate, sodium potassium tartrate, sodium hydrogen arsenate, sodium phosphate, ammonium molybdate, phenol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), aluminum chloride, silver nitrate (AgNO3), sodium nitrite, Folin–Ciocalteu reagent, Conc. sulfuric acid, catechin, nutrient agar (NA), and potato dextrose broth (PDB). The strains of Staphylococcus aureus (MTCC 96), Xanthomonas spp. (MTCC 11102), Fusarium oxysporum (MTCC 284), and Macrophomina phaseolina (MTCC 10399) were obtained from the Institute of Microbial Technology (IMTECH), Chandigarh, India’s culture collecting center for microbial resources. For the evaluation of antimicrobial activity, experiments were conducted in the department of microbiology, CCS HAU Hisar.
Trigonella foenum-graecum L. leaves of the Hisar Mukta (HM) 425 variety were procured from the Chaudhary Charan Singh Haryana Agricultural University’s Vegetable Science Research Farm. The collected leaf samples were verified by Dr. Anita, Assistant professor, Department of Botany and Plant physiology, CCS HAU, Hisar, India, by using an online platform (Tropicos and IPNI). The voucher specimens were verified by the Medicinal, Aromatic and Potential Crops Section, Department of Genetics and Plant Breeding, CCS HAU Hisar, by voucher specimen number 20.
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