Monocyte Isolation and Differentiation

Monocytes were enriched from peripheral blood mononuclear cells from three healthy donors using Rossette Sep Monocyte enrichment cocktail through Ficoll separation (STEMCELL Technologies, Vancouver, Canada) as previously described (22 (link)). Purified monocytes were then differentiated in X-vivo15 medium (Lonza, Walkersville, MD, USA), with GMCSF 1000U/ml and IL4 1000U/ml (Pepro Tech Inc, Rocky Hill, NJ, USA) with the change of media every other day.

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Elsayed R., Kurago Z., Cutler C.W., Arce R., Gerber J., Celis E., Sultan H., Elashiry M., Meghil M., Sun C., Auersvald C.M., Elsayed R., Elshikh M.E., Isales C, & Elsalanty M.E. (2020). Role of dendritic cell - mediated immune response in oral homeostasis: a new mechanism of osteonecrosis of the jaw. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(2), 2595-2608.

Publication 2020

Ficoll separation X-vivo15 medium GMCSF IL4

Donors Ficoll Gmcsf Monocytes Peripheral blood mononuclear cells Stemcell

Corresponding Organization :

Other organizations : Augusta University, Washington University in St. Louis, William Harvey Research Institute

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Variable analysis

independent variables

Rossette Sep Monocyte enrichment cocktail
GMCSF 1000U/ml
IL4 1000U/ml

dependent variables

Purified monocyte differentiation

control variables

Peripheral blood mononuclear cells from three healthy donors
X-vivo15 medium
Ficoll separation

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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