Protocol detail

LPS-Induced Behavioral Changes in Mice

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LPS from E. coli serotype O26:B6 was obtained from Sigma Aldrich (St. Louis, MO, USA). The LPS was dissolved in sterile saline vehicle to a concentration of 0.1mg/ml. Mice were randomly assigned to treatment groups and received a single intraperitoneal injection of LPS (1.5mg/kg body weight) or an equivalent volume of sterile saline vehicle and returned to their home cage immediately following injection. The dose of LPS when administered during the peripubertal period has previously been demonstrated to decrease behavioral responses to estradiol in adulthood [8 (link), 10 (link), 18 (link), 19 , 32 (link)]. Consistent with previous reports [8 (link), 10 (link), 18 (link), 19 , 32 (link)], this dose of LPS produced moderate sickness behavior.

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Holder M.K, & Blaustein J.D. (2017). Developmental time course and effects of immunostressors that alter hormone-responsive behavior on microglia in the peripubertal and adult female mouse brain. PLoS ONE, 12(2), e0171381.

Publication 2017

LPS from E. coli serotype O26:B6

Body weight E coli Estradiol Intraperitoneal injection Mice Saline Sterile

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Variable analysis

independent variables

LPS injection (1.5mg/kg body weight) or sterile saline vehicle

dependent variables

Behavioral responses to estradiol in adulthood

control variables

Mice were randomly assigned to treatment groups
Sterile saline vehicle as a control

controls

Sterile saline vehicle as a negative control

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