Synthesis and Purification of TP4 Peptides

TP4 (H-FIHHIIGGLFSAGKAIHRLIRRRRR-OH) and TP4 biotinylated at the N-terminus were synthesized and purified by GL Biochem Ltd. (Shanghai, China) as previously described [23 (link), 41 (link)]. Autocamtide-2 related inhibitory peptide II (AIP II) and PD98059 were purchased from EMD Millipore. BAPTA-AM [1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)], Paclitaxel, Docetaxel, Epirubicin hydrochloride, and Doxorubicin hydrochloride were purchased from Sigma.

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Ting C.H., Chen Y.C., Wu C.J, & Chen J.Y. (2016). Targeting FOSB with a cationic antimicrobial peptide, TP4, for treatment of triple-negative breast cancer. Oncotarget, 7(26), 40329-40347.

Publication 2016

PD98059 BAPTA-AM Paclitaxel Docetaxel Epirubicin hydrochloride Doxorubicin hydrochloride

Acid Autocamtide 2 related inhibitory peptide Bapta Docetaxel Doxorubicin hydrochloride Epirubicin hydrochloride Ester Ethane Paclitaxel Pd98059

Corresponding Organization :

Other organizations : Institute of Cellular and Organismic Biology, Academia Sinica, National Taiwan Ocean University

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Variable analysis

independent variables

TP4 (H-FIHHIIGGLFSAGKAIHRLIRRRRR-OH)
TP4 biotinylated at the N-terminus
Autocamtide-2 related inhibitory peptide II (AIP II)
PD98059
BAPTA-AM [1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)]
Paclitaxel
Docetaxel
Epirubicin hydrochloride
Doxorubicin hydrochloride

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Not explicitly mentioned

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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