Tongue Tumor Xenograft Mouse Model

CAL27 cells (3 x 10⁵) were injected into the tongues of six-week old female NCr nude mice (CrTac:NCr-Foxn1^nu, Taconic, Hudson, NY) as we previously described [32 (link)]. Mice began a daily lavage schedule with 50 μl sesame oil alone or CB7993113 dissolved in sesame oil (50 mg/kg/day in 50 μl) 24 hours before tongue injections. Animals were weighed and tumor size was measured with Vernier calipers over a 26 day period. Animals were sacrificed when mice lost 20% of their total body mass. Surviving mice were sacrificed by day 26. Animals were housed at the Association for Assessment and Accreditation of Laboratory Animal Care-certified Boston University Medical Laboratory Animal Science Center and used in accordance with the NIH Guide for the Care and Use of Laboratory Animals. A Boston University Medical Campus Institutional Animal Care and Use Committee-approved protocol was followed.