All animal studies were approved by the LSU Health Sciences Center–Shreveport institutional animal care and use committee. All animals were cared for according to the National Institutes of Health guidelines for the care and use of laboratory animals.
Mice lacking lipin-1 enzymatic activity from myeloid cells (lipin-1mEnzyKO) were generated as previously reported (19 (link)). Briefly, mice with exons 3 and 4 of the Lpin1 gene flanked by LoxP sites (genetic background: C57BL/6J and SV129; generously provided by B.N.F. and R. Chrast) were crossed with C57BL/6J LysM-Cre transgenic mice purchased from The Jackson Laboratory (Bar Harbor, ME). Mice fully lacking lipin-1 from myeloid cells (lipin-1mKO) were generated by crossing mice with exon 7 of the Lpin1 gene flanked by LoxP sites (genetic background: C57BL/6J and SV129; generously provided by B.N.F. (23 (link)) with C57BL/6J LysM-Cre transgenic mice purchased from The Jackson Laboratory. Age-matched lipin-1 flox/flox littermate mice were used as controls.
Mice lacking lipin-1 enzymatic activity from myeloid cells (lipin-1mEnzyKO) were generated as previously reported (19 (link)). Briefly, mice with exons 3 and 4 of the Lpin1 gene flanked by LoxP sites (genetic background: C57BL/6J and SV129; generously provided by B.N.F. and R. Chrast) were crossed with C57BL/6J LysM-Cre transgenic mice purchased from The Jackson Laboratory (Bar Harbor, ME). Mice fully lacking lipin-1 from myeloid cells (lipin-1mKO) were generated by crossing mice with exon 7 of the Lpin1 gene flanked by LoxP sites (genetic background: C57BL/6J and SV129; generously provided by B.N.F. (23 (link)) with C57BL/6J LysM-Cre transgenic mice purchased from The Jackson Laboratory. Age-matched lipin-1 flox/flox littermate mice were used as controls.
