Imaging and Tracking Microglial Dynamics

Images were taken with a Cell Observer CSU-X1 Yokogawa Spinning Disk, AxioCam MRm and Evolve 512 (Zeiss). For in vivo imaging 1.5dpf embryos or 3dpf/5dpf larvae were anesthetized in E3 1xTricaine (80 mg/l) (Pharmaq Ltd) and were mounted in 1.5% agarose (Invitrogen) dissolved in E3 1xTricaine. Embedded embryos were covered with E3 1xTricaine. Brightness and contrast were adjusted using Zen blue (Zeiss) and ImageJ. Image stitching was performed with the ImageJ plugin Image stitching [69 (link)] and microglia were tracked with the ImageJ plugin mTrackJ [70 (link)].

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Solchenberger B., Russell C., Kremmer E., Haass C, & Schmid B. (2015). Granulin Knock Out Zebrafish Lack Frontotemporal Lobar Degeneration and Neuronal Ceroid Lipofuscinosis Pathology. PLoS ONE, 10(3), e0118956.

Publication 2015

AxioCam MRm Evolve 512 agarose Zen blue

Agarose Cell Embryos Larvae Microglia

Corresponding Organization : Munich Cluster for Systems Neurology

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Variable analysis

independent variables

Embryo developmental stage (1.5dpf, 3dpf, 5dpf)

dependent variables

Microglia tracking and movement

control variables

Anesthesia (E3 1xTricaine, 80 mg/l)
Mounting medium (1.5% agarose in E3 1xTricaine)
Imaging equipment (Cell Observer CSU-X1 Yokogawa Spinning Disk, AxioCam MRm, Evolve 512)
Image processing (Brightness and contrast adjustment, Image stitching, Microglia tracking)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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