Evaluation of Candidate Reference Genes in Whitefly Transcriptomics

Housekeeping genes from a previous B. tabaci transcriptomic study [29] (link) were selected as candidate reference genes including β-actin (Actin), 18S rRNA (18S), heat shock protein (HSP20, HSP40, HSP70, HSP90), γ-tubulin, 60S ribosomal protein L29 (RPL29), succinate dehydrogenase complex subunit A (SDHA), flavoprotein, glyceraldehyde phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF-1α), peptidylprolyl isomeraseA (PPIA), NADH dehydrogenase (NADH), Myosin light chain (Myosin L), and adenosine triphosphate enzyme (ATPase). Primer 5.0 (http://www.premierbiosoft.com/) was used to design primers for qRT-PCR analysis. The validity of these candidate reference genes were evaluated under selected biotic and abiotic conditions described in the following sections.

Free full text: Click here

Li R., Xie W., Wang S., Wu Q., Yang N., Yang X., Pan H., Zhou X., Bai L., Xu B., Zhou X, & Zhang Y. (2013). Reference Gene Selection for qRT-PCR Analysis in the Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). PLoS ONE, 8(1), e53006.

Publication 2013

18s rrna 60s ribosomal protein l29 Actin Adenosine triphosphate Elongation factor 1 alpha Enzyme Flavoprotein Genes Glyceraldehyde phosphate dehydrogenase Heat shock protein Housekeeping genes Hsp40 Hsp70 Hsp90 Myosin Myosin light chain Nadh Nadh dehydrogenase Primers Subunit Succinate dehydrogenase Transcriptomic Tubulin

Corresponding Organization : University of Kentucky

Top 5 similar protocols

Protocol cited in 16 other protocols

Variable analysis

independent variables

Selected biotic and abiotic conditions

dependent variables

Validity of candidate reference genes

control variables

Candidate reference genes including β-actin (Actin), 18S rRNA (18S), heat shock protein (HSP20, HSP40, HSP70, HSP90), γ-tubulin, 60S ribosomal protein L29 (RPL29), succinate dehydrogenase complex subunit A (SDHA), flavoprotein, glyceraldehyde phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF-1α), peptidylprolyl isomeraseA (PPIA), NADH dehydrogenase (NADH), Myosin light chain (Myosin L), and adenosine triphosphate enzyme (ATPase)

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!