Approximately 100 gm of each dried powder were separately extracted using 500 ml of petroleum ether, dichloromethane and methanol. Maceration with stirring was done for 72 h at room temperature. The mixture was filtered through Whatman No.1 filter paper. The extracts were then concentrated at 40 °C (for petroleum ether and dichloromethane extracts) and at 65 °C (for methanol extracts) using a rotary evaporator (Heidolph WB2000, Germany) [27 (link)]. The organic solvent extracts were further evaporated to dryness at 40 °C in an oven before storing at 4 °C for future use [28 (link)].
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