To obtain leukocyte and RBC counts, 50 μl of PB was taken from the retro-orbital plexus of the mice and collected in microvette EDTA-coated tubes (Sarstedt Inc., Newton, NC, USA), and samples were analyzed within 2 h of collection on a HemaVet 950 analyzer (Drew Scientific Inc., Waterbury, CT, USA) [35 (link), 44 (link), 45 (link)].
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