Antibody Sources for Cell Signaling

The primary antibodies of TEAD1 (sc-376113), TEAD4 (sc-134071), and CTGF (L-20) (sc-14939) were from Santa Cruz (Dallas, TX, USA). YAP1 (ab52771) antibody and HA tag (ab18181) were achieved from Abcam (Cambridge, MA, USA). AMOTL1 (HPA001196) and Flag-tag (F3165) antibodies were obtained from Sigma-Aldrich (St. Louis, MO, USA). Other primary antibodies were from Cell Signaling (Danvers, MA, USA), including p21 (#2946), p27 (#2552), pRb (Ser807/811) (#9308), Erk (#9102), pErk (#9101), Myc (#2278), cyclin D1 (#2978), c-Myc (#9402), and GAPDH (#2118). Anti-Mouse IgG-HRP (Dako, Glostrup, Denmark, 00049039, 1:30,000) and anti-Rabbit IgG-HRP (Dako, 00028856, 1:10,000) were used for secondary antibodies. The related protocol was suggested before [13 (link)].

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Zhou Y., Zhang J., Li H., Huang T., Wong C.C., Wu F., Wu M., Weng N., Liu L., Cheng A.S., Yu J., Wong N., Lo K.W., Tang P.M., Kang W, & To K.F. (2020). AMOTL1 enhances YAP1 stability and promotes YAP1-driven gastric oncogenesis. Oncogene, 39(22), 4375-4389.

Publication 2020

YAP1 (ab52771) antibody AMOTL1 (HPA001196) Flag-tag (F3165) pRb (Ser807/811) pErk cyclin D1 c-Myc GAPDH Anti-Mouse IgG-HRP anti-Rabbit IgG-HRP

Anti igg Antibodies Antibody C myc Ctgf Cyclin d1 Gapdh Mouse Rabbit Yap1

Corresponding Organization :

Other organizations : Chinese University of Hong Kong, Prince of Wales Hospital, Sun Yat-sen University Cancer Center, Sun Yat-sen University, Jinan University

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Variable analysis

independent variables

Primary antibodies for TEAD1 (sc-376113), TEAD4 (sc-134071), CTGF (L-20) (sc-14939), YAP1 (ab52771), HA tag (ab18181), AMOTL1 (HPA001196), and Flag-tag (F3165)

dependent variables

Not explicitly mentioned

control variables

Anti-Mouse IgG-HRP (Dako, Glostrup, Denmark, 00049039, 1:30,000) and anti-Rabbit IgG-HRP (Dako, 00028856, 1:10,000) as secondary antibodies
The related protocol was suggested before [13 (link)]

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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