Stereological Quantification of Midbrain Cells

The number of TH- and GFAP-positive cells in the substantia nigra was estimated using a random sampling stereological counting method. Images were sampled from at least four different points within each substantia nigra section [37 (link)]. TH (ab6211), GFAP (ab7260), and DAPI (ab104139) were from Abcam, USA. All immunoreactive cells were counted regardless of the intensity of labeling. The slides were photographed with a fluorescence microscope (Nikon, Japan).

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Lei K., Shen Y., He Y., Zhang L., Zhang J., Tong W., Xu Y, & Jin L. (2020). Baicalin Represses C/EBPβ via Its Antioxidative Effect in Parkinson's Disease. Oxidative Medicine and Cellular Longevity, 2020, 8951907.

Publication 2020

ab6211 fluorescence microscope

Cells Dapi Fluorescence microscope Gfap Substantia nigra

Corresponding Organization : Tongji Hospital

Other organizations : Emory University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

The number of TH-positive cells in the substantia nigra
The number of GFAP-positive cells in the substantia nigra

control variables

Substantia nigra sections
Sampling points within each substantia nigra section
TH (ab6211), GFAP (ab7260), and DAPI (ab104139) antibodies from Abcam, USA
Fluorescence microscope (Nikon, Japan)

controls

None explicitly mentioned

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